Our History

American Chestnut Restoration Efforts

Late 1800s

The Arrival of Chestnut Blight

  • The chestnut blight was an unforeseen consequence of importing Asian chestnuts, which had evolved alongside the fungus and developed resistance. American chestnuts, on the other hand, had no defense against it, and by the early 1900s, the fungus had spread through vast forests. The American chestnut, once a dominant species, was decimated over the following decades. The introduction of the disease reshaped entire ecosystems, as the chestnut had played a crucial role in the food chain for wildlife and humans.

1904

The Discovery of Blight

  • The discovery of the blight was a significant turning point. The fungus caused tree trunks to die, and while chestnuts would sprout from the base of the tree, they too were quickly infected and killed. Over time, only a few mature chestnuts remained, as the blight continued to devastate the species for over 100 years.

1950

The American Chestnut is Nearly Wiped Out

  • In the mid-20th century, the American chestnut had essentially vanished from its native forests. Even though some trees continued to sprout from the remaining roots, the blight's repetitive damage prevented them from maturing and reproducing. As a result, the species faced local extinction across much of its range, especially in the Appalachian Mountains, where it had once been most abundant.

1983

The American Chestnut Foundation (TACF) is Founded

  • TACF's backcross breeding approach aimed to combine the blight resistance of Chinese chestnuts with the genetic traits of American chestnuts. The process involved multiple generations of crossing, which eventually created hybrid trees with a blend of characteristics from both species. While promising, this approach also faced challenges in retaining the American chestnut's genetic purity.

1988

A Discovery in Zoar Valley Inspires a Movement

  • Herb and Jane Darling, from Buffalo, NY, first encountered American chestnuts when a friend hunting on their Zoar Valley property discovered a massive 80-foot-tall tree with a 24-inch diameter at breast height. This rare survivor, which had thus far escaped the blight, sparked Herb's deep fascination with the species. Herb later recalled: “The discovery of the large American chestnut on my property was the original inspiration which caused me to search and find TACF and to join.”

1988

The New York State Chestnut Foundation is Established and Initiates Collaboration with ESF

  • Rather than relying on traditional breeding methods, they proposed a bold new approach: using biotechnology to develop a chestnut tree resistant to blight. To support this vision, they established the New York State Chestnut Foundation, dedicated to advancing transgenic research in chestnut restoration.

    The partnership with ESF took a major step forward when they joined forces with researchers Dr. William Powell and Dr. Charles Maynard, who were already working on chestnut restoration. Together, they focused on developing a genetically engineered American chestnut that could resist blight without relying on genes from the Chinese chestnut. This innovative approach marked a significant turning point in the restoration effort and laid the groundwork for the creation of transgenic American chestnut trees.

1989

NY-TACF Becomes the First Chapter of TACF

  • TACF invited the New York State Chestnut Foundation to become its first official chapter, solidifying NY-TACF's role in the broader restoration movement. The chapter’s focus on biotechnology, combined with TACF’s traditional breeding program, created a unique dual approach to restoration. While TACF concentrated on conventional breeding techniques, NY-TACF remained committed to advancing the genetic approach, with significant support from ESF's pioneering research.

1997

First Non-Transgenic Tissue Culture Plantlets are Planted

  • Tissue culture is a laboratory technique that involves growing plant cells or tissues in a controlled environment to produce new plants. For the chestnut restoration effort, this method was crucial in improving the survival rate of young trees. The plantlets were developed from embryogenic cell lines, which have the ability to generate entire plants, and then planted in the field. While these plantlets were non-transgenic, they demonstrated improved rooting and acclimatization techniques, which enhanced their ability to survive in natural conditions. This work laid a strong foundation for both traditional and transgenic restoration approaches, contributing significantly to the overall effort.

2001

Oxalate Oxidase (OxO) Testing and First Transgenic Experiments

  • The OxO gene from wheat was initially tested in hybrid poplars and showed promising results in increasing resistance to blight. The gene works by breaking down oxalic acid, a toxin produced by the chestnut blight fungus that weakens the tree’s defenses. This discovery paved the way for genetic engineering efforts aimed at introducing the OxO gene into American chestnuts, marking a key step in the development of transgenic trees.

2006

First Transgenic American Chestnut Trees Planted in the Field

  • The introduction of this genetic modification demonstrated the potential of biotechnology to combat chestnut blight and offered new hope for the restoration of American chestnuts to their native habitats. While these trees were not fully resistant to the disease, the field trials proved invaluable, providing key insights into the effectiveness of the genetic modifications in real-world conditions. This marked the beginning of a new phase in the restoration effort and paved the way for future advancements in transgenic chestnut research.

2015

Introduction of Darling Tree: The First Blight-Tolerant American Chestnut

  • The introduction of the Darling tree was a historic moment in the American chestnut restoration initiative. As the first genetically modified American chestnut with genuine blight tolerance, Darling proved that the OxO gene could allow the tree to survive and thrive in its native ecosystem. This breakthrough renewed hope for the species' restoration and ignited further research into transgenic chestnuts.

2015

Early Flowering in Chestnut Species Induced Under High-Dose Light

  • The study, titled "Early flowering in chestnut species induced under high-dose light in growth chambers," explored how different light intensities impact the developmental timing of chestnut trees. By growing seedlings in controlled environments with elevated light levels, the researchers observed that these conditions accelerated the flowering process compared to standard lighting. This discovery highlights the potential for using light manipulation to control and possibly optimize flowering schedules in chestnut breeding and cultivation programs, offering valuable strategies for growers and researchers.

2015–2019

A Breakthrough in Blight-Tolerant American Chestnut Breeding

  • In 2015, ESF researchers demonstrated that high-intensity light could induce early male and female flowering in chestnut trees, with male flowers appearing in under a year. This breakthrough shortened breeding cycles and laid the foundation for faster production of resistant seedlings.

    Building on this in 2019, Dr. Klak at UNE produced large quantities of transgenic pollen from OxO-expressing trees, leading to the generation of thousands of transgenic nuts through controlled pollination. UNE also successfully grew mature seeds indoors, resulting in new blight-resistant plants.

    More recently, ESF and UNE achieved successful crosses of transgenic plants under high-intensity light, producing homozygous trees carrying two copies of the OxO gene. These trees yield 100% OxO-carrying pollen, ensuring that all resulting nuts inherit blight resistance—marking a significant step toward restoring the American chestnut.

2018

Development of a Rapid Blight Resistance Test

  • The assay involves applying the chestnut blight fungus directly to detached chestnut leaves and measuring the resulting lesion size. These measurements reliably correlate with traditional stem inoculation results but require less time, space, and resources. This method enables rapid, large-scale screening of seedlings, making it a key tool in restoring blight-tolerant American chestnuts. 

2018

Publication on Tadpoles, Fungi, and Bumblebees

  • The publication in 2018 was part of a broader effort to assess the environmental impact of transgenic chestnuts. Researchers explored how the Darling tree might affect local ecosystems, including aquatic life like tadpoles, soil fungi, and pollinators like bumblebees. These studies were essential for addressing concerns about the ecological safety of planting genetically modified trees in natural habitats and provided data that could support regulatory approval.

2019

Research Breakthroughs Continue

  • In 2019, significant breakthroughs in transgenic chestnut research were made. New gene transformations were developed to further improve blight resistance and the overall health of the trees. These advancements also focused on refining planting techniques, which increased the success rates of transgenic chestnuts in field trials. The ongoing research at ESF contributed to improving the viability of restoring the American chestnut to its native habitat.

2020

ESF Designates Chestnut Restoration Forest

  • The creation of the Chestnut Restoration Demonstration Forest at ESF marked a significant milestone in practical restoration efforts. This 8-acre site serves as a living laboratory where scientists can test planting methods, tree care strategies, and long-term growth patterns of American chestnuts, including transgenic varieties. It provides an opportunity to observe how restored chestnuts might adapt to real-world environmental conditions, advancing the efforts to reintroduce the species into U.S. forests.

    Dedication to Bill Powell

    In the fall of 2024, the site was dedicated to Dr. Bill Powell, a pioneer in American chestnut restoration, who passed away before the tree was officially approved. Dr. Powell’s contributions to the development of genetically modified chestnuts, particularly his work with the Darling tree and the incorporation of the OxO gene, were instrumental in advancing the restoration effort. The dedication honored his legacy and the significant impact he made in the field of forest restoration.

2021

Deregulation Process at ESF

  • The deregulation process involves comprehensive evaluations of the tree's environmental safety and potential impacts on native ecosystems. If successful, it will clear the way for large-scale restoration efforts, bringing the American chestnut closer to recovery in its natural environment.

2025

NY-TACF Becomes American Chestnut Restoration, Inc. (ACR)

  • The rebranding of NY-TACF to American Chestnut Restoration, Inc. (ACR) marks a new chapter in the organization’s history. With TACF’s decision to discontinue support for ESF’s transgenic chestnut research, ACR remains dedicated to advancing the development of blight-tolerant chestnuts. The organization continues its collaboration with ESF, focusing on further developing and field-testing the Darling and DarWin transgenic chestnut varieties. ACR also advocates for federal approval to allow for wider distribution of these trees.